| Cell lines- in vivo biotinylation |
| AVB101 |
| AVB101- glycerol stock |
| CVB101- chemically competent cells |
| EVB101- electro-competent cells |
| AVB100 |
| AVB100- glycerol stock |
| EVB100- electro-competent cells |
| AVB99 |
| AVB99-glycerol stock |
| BIP-300- positive control plasmid for induction |
| |
| Enzyme- in vitro biotinylation |
| Biotin ligase |
| BirA500- 40ug biotin ligase |
| Bulk BirA- 300 ug biotin ligase |
| Large quantities of biotin ligase |
| BIS-300 positive and negative control protein kit |
| BIO-200 biotin solution |
| BIO-500 biotin solution |
| |
| Vectors containing AviTag |
| C-terminus Avitag |
| pAC4 |
| AC5 |
| pAC6 |
| All three |
| Sequencing primers |
| N-terminus Avitag |
| pAN4 |
| pAN5 |
| pAN6 |
| All three |
| Sequencing primers |
| |
| Purified Biotinylated Proteins and support chemicals |
| Gaussia princeps Luciferase |
| Gluc-10 |
| Gluc-100 |
| Renilla reniformis Green Fluorescent Protein |
| Streptavidin-conjugated proteins |
| Streptavidin-Gaussia princeps Luciferase |
| Streptavidin-Renilla reniformis GFP |
| Coelenterazine |
| |
| Purification and Identification of Avitag'd proteins |
| AbC- antibody against C-terminus Avitag |
| AbCA -Abc antibody conjugated to agarose |
| BIS-300 positive and negative control protein kit |
